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Charite Research Organisation ang ii type 1 (at1) receptors
Ang Ii Type 1 (At1) Receptors, supplied by Charite Research Organisation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5. Representative western blots gel image and mean values of <t>AT1R</t> (A) and Mas receptor (B) protein expressions within the PVN in WT and Akita mice (n = 6–7/group). Values are presented as mean ± SE. *P < 0.05 compared with WT.
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Fig. 5. Representative western blots gel image and mean values of <t>AT1R</t> (A) and Mas receptor (B) protein expressions within the PVN in WT and Akita mice (n = 6–7/group). Values are presented as mean ± SE. *P < 0.05 compared with WT.
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Fig. 5. Representative western blots gel image and mean values of <t>AT1R</t> (A) and Mas receptor (B) protein expressions within the PVN in WT and Akita mice (n = 6–7/group). Values are presented as mean ± SE. *P < 0.05 compared with WT.
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Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and <t>Ang</t> <t>II</t> level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.
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Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and <t>Ang</t> <t>II</t> level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.
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Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and <t>Ang</t> <t>II</t> level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.
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Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and <t>Ang</t> <t>II</t> level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.
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Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and <t>Ang</t> <t>II</t> level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.
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Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and <t>Ang</t> <t>II</t> level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.
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Image Search Results


Fig. 5. Representative western blots gel image and mean values of AT1R (A) and Mas receptor (B) protein expressions within the PVN in WT and Akita mice (n = 6–7/group). Values are presented as mean ± SE. *P < 0.05 compared with WT.

Journal: Nitric oxide : biology and chemistry

Article Title: Downregulation of neuronal nitric oxide synthase (nNOS) within the paraventricular nucleus in Ins2 Akita -type-1 diabetic mice contributes to sympatho-excitation.

doi: 10.1016/j.niox.2024.11.001

Figure Lengend Snippet: Fig. 5. Representative western blots gel image and mean values of AT1R (A) and Mas receptor (B) protein expressions within the PVN in WT and Akita mice (n = 6–7/group). Values are presented as mean ± SE. *P < 0.05 compared with WT.

Article Snippet: The following primary antibodies were used: nNOS (sc-5302, 1:500), ACE (sc-23908, 1:250), ACE2 (sc-20998, 1:250), Ang II type 1 receptor (AT1R) (sc-515884, 1:250), Mas receptor (sc-390453, 1:250), protein inhibitor of nNOS (PIN) (sc-13969, 1:500), and β-actin (sc-47778, 1:1000) (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Western Blot

Fig. 7. Proposed model for the up-regulation of the PIN by post-translational regulation in the PVN. Elevated central Ang II levels via AT1R in the PVN increase the expression of PIN via decreasing the ubiquitination. Increased expression of PIN destabilizes nNOS dimers, which renders nNOS catalytically inactive, either by interfering with the assembly or dimer stability. A reduced level of functional nNOS reduces NO production in the PVN, causing an increase in sympatho-excitation and associated blood pressure.

Journal: Nitric oxide : biology and chemistry

Article Title: Downregulation of neuronal nitric oxide synthase (nNOS) within the paraventricular nucleus in Ins2 Akita -type-1 diabetic mice contributes to sympatho-excitation.

doi: 10.1016/j.niox.2024.11.001

Figure Lengend Snippet: Fig. 7. Proposed model for the up-regulation of the PIN by post-translational regulation in the PVN. Elevated central Ang II levels via AT1R in the PVN increase the expression of PIN via decreasing the ubiquitination. Increased expression of PIN destabilizes nNOS dimers, which renders nNOS catalytically inactive, either by interfering with the assembly or dimer stability. A reduced level of functional nNOS reduces NO production in the PVN, causing an increase in sympatho-excitation and associated blood pressure.

Article Snippet: The following primary antibodies were used: nNOS (sc-5302, 1:500), ACE (sc-23908, 1:250), ACE2 (sc-20998, 1:250), Ang II type 1 receptor (AT1R) (sc-515884, 1:250), Mas receptor (sc-390453, 1:250), protein inhibitor of nNOS (PIN) (sc-13969, 1:500), and β-actin (sc-47778, 1:1000) (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Expressing, Ubiquitin Proteomics, Functional Assay

Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and Ang II level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.

Journal: Acta Pharmacologica Sinica

Article Title: Gut microbiota dysbiosis-induced activation of the intrarenal renin–angiotensin system is involved in kidney injuries in rat diabetic nephropathy

doi: 10.1038/s41401-019-0326-5

Figure Lengend Snippet: Intrarenal RAS is activated in early DN. Plasma renin activity level ( a ), Ang I level ( b ), and Ang II level ( c ). The protein expression levels of RAS were measured by Western blotting ( d ). The histograms represent the mean ± SD of the densitometric scans of the protein bands normalized to β-actin ( e ). Correlation analysis of plasma acetate levels and intrarenal Ang II expression levels ( f ) ( r = 0.969, P < 0.001). * P < 0.01 compared with the control group, # P < 0.05 compared with the DM group.

Article Snippet: The membranes were then immersed in blocking buffer for 1 h at room temperature and incubated overnight at 4 °C in primary antibodies against angiotensin-converting enzyme (ACE), angiotensinogen (AGT), Ang II, and Ang II type 1 receptor (AT1) (Santa Cruz, USA).

Techniques: Clinical Proteomics, Activity Assay, Expressing, Western Blot, Control